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pET15b_LwaCas13a-6xHIS
(Plasmid #251214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251214 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LwaCas13a
  • Species
    Leptotrichia wadei
  • Promoter lac
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    LwaCas13a was a gift from Feng Zhang (Addgene plasmid #91909).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b_LwaCas13a-6xHIS was a gift from Niels Geijsen (Addgene plasmid # 251214 ; http://n2t.net/addgene:251214 ; RRID:Addgene_251214)
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