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pMTL-YN1C Promoter-less mNeonGreen
(Plasmid #251223)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251223 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMTL-YN1c
  • Backbone size w/o insert (bp) 6064
  • Total vector size (bp) 6852
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Leader peptide with mNeonGreen codon optimized for C. difficile
  • Species
    C. difficile
  • Insert Size (bp)
    788
  • Promoter Promoter-less

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctgatgagtgtcctttatgtaagg
  • 3′ sequencing primer taacgccagggttttccc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTL-YN1C Promoter-less mNeonGreen was a gift from Aimee Shen (Addgene plasmid # 251223 ; http://n2t.net/addgene:251223 ; RRID:Addgene_251223)
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