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CYLBL_Neo_TREdCas9-KRAB
(Plasmid #251231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251231 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CYLBL_Neo_CAGdCas9-KRAB
  • Backbone manufacturer
    Addgene_220439
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 12136
  • Modifications to backbone
    original CLYBL_Neo_CAGdCas9-KRAB was obtained from Yin Shen's lab. It was modified to change CAG promoter to TRE and selection cassette from Neo to PTK.
  • Vector type
    Mammalian Expression, CRISPR, TALEN
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRE-dCas9 KRAB
  • Species
    Synthetic
  • Insert Size (bp)
    5377
  • Mutation
    D10A and H840A
  • Promoter TRE
  • Tag / Fusion Protein
    • KRAB (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Obtained insert from Yin Shen, Addgene plasmid 220439

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CYLBL_Neo_TREdCas9-KRAB was a gift from Gary Hon (Addgene plasmid # 251231 ; http://n2t.net/addgene:251231 ; RRID:Addgene_251231)
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