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Corona_Wuhan_RBD
(Plasmid #251256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251256 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Corona_Wuhan_RBD
  • Species
    SARS-CoV-2 Wuhan variant
  • Mutation
    C9A
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Corona_Wuhan_RBD was a gift from Yutaka Kuroda (Addgene plasmid # 251256 ; http://n2t.net/addgene:251256 ; RRID:Addgene_251256)

  • For your References section:

    A Multi-Disulfide Receptor-Binding Domain (RBD) of the SARS-CoV-2 Spike Protein Expressed in E. coli Using a SEP-Tag Produces Antisera Interacting with the Mammalian Cell Expressed Spike (S1) Protein. Brindha S, Kuroda Y. Int J Mol Sci. 2022 Feb 1;23(3):1703. doi: 10.3390/ijms23031703. 10.3390/ijms23031703 PubMed 35163624
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