scAAV-DDIO-DTA
(Plasmid
#251347)
-
PurposeCre and Flp dependent DTA (diptheria toxin) expressing system
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonescAAV1-hSyn:FLEX:eGFP
-
Vector typeMammalian Expression, Bacterial Expression, AAV, Cre/Lox
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCre/Flp Dependent DTA driven by a EF1α(core) promoter
-
SpeciesC. diphtheriae
-
Insert Size (bp)1800
- Promoter EF1α
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gggcagagcgcacatcg
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.18.683193 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
scAAV-DDIO-DTA was a gift from Samuel Pfaff (Addgene plasmid # 251347 ; http://n2t.net/addgene:251347 ; RRID:Addgene_251347) -
For your References section:
A genetically-defined population of amygdalofugal neurons promotes suckling and early postnatal growth. Moore JD, Bachmann LC, McElvain LE, Pfaff SL, Dulac C. bioRxiv [Preprint]. 2025 Oct 18:2025.10.18.683193. doi: 10.1101/2025.10.18.683193. 10.1101/2025.10.18.683193 PubMed 41279177