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SFFV-ZIM3-dCas9-2A-mCherry
(Plasmid #251398)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251398 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    LentiV
  • Total vector size (bp) 11865
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZIM3
  • Species
    Synthetic
  • Mutation
    Includes ZIM3 aa 1-100
  • Entrez Gene
    ZIM3 (a.k.a. ZNF657)
  • Promoter SFFV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SFFV-ZIM3-dCas9-2A-mCherry was a gift from Junwei Shi (Addgene plasmid # 251398 ; http://n2t.net/addgene:251398 ; RRID:Addgene_251398)

  • For your References section:

    CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samples. Cao Z, Yu S, Peng J, Barrett DR, Liu Y, Sussman JH, Chen C, Thadi A, Liu L, Alikarami F, Xu J, Carroll MP, Tan K, Bernt KM, Shi J. Mol Cell. 2026 Mar 5;86(5):968-985.e7. doi: 10.1016/j.molcel.2026.02.003. Epub 2026 Feb 26. 10.1016/j.molcel.2026.02.003 PubMed 41759529
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