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pHalo2X-mMYO7A-RK/AA
(Plasmid #251426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251426 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHalo2X-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myosin VIIa (RK/AA)
  • Species
    M. musculus (mouse)
  • Mutation
    Two missense mutations in the RGSK motif of the second MyTH4FERM domain
  • Entrez Gene
    Myo7a (a.k.a. Hdb, Myo7, USH1B, nmf371, polka, sh-1, sh1)
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is derived from the Clontech pEGFP-C1 vector. The XhoI and SalI sites in the original HaloTag sequence were disrupted by silent mutations. Two missense mutations, p.R2127A and p.K2130A, were introduced into MYO7A (NP_001243012.1).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHalo2X-mMYO7A-RK/AA was a gift from Thomas Friedman & Takushi Miyoshi (Addgene plasmid # 251426 ; http://n2t.net/addgene:251426 ; RRID:Addgene_251426)

  • For your References section:

    Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells. Miyoshi T, Vishwasrao HD, Belyantseva IA, Miyoshi J, Sajeevadathan M, Ishibashi Y, Adadey SM, Harada N, Shroff H, Friedman TB. Nat Commun. 2025 Sep 1;16(1):8149. doi: 10.1038/s41467-025-63102-0. 10.1038/s41467-025-63102-0 PubMed 40890108
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