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FRB-mUSH1C-DFCR-pEGFP
(Plasmid #251431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251431 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    The DFCR fragment (residues 296-728 of Ush1C)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ush1c (a.k.a. 2010016F01Rik, harmonin)
  • Tags / Fusion Proteins
    • FRB (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is derived from the Clontech pEGFP-N3 vector. FKBP undergoes AP21987-dependent heterodimerization with FRB. The actin-binding motif of harmonin b corresponds to amino acids 296-729 of NP_001157205.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-mUSH1C-DFCR-pEGFP was a gift from Thomas Friedman & Takushi Miyoshi (Addgene plasmid # 251431 ; http://n2t.net/addgene:251431 ; RRID:Addgene_251431)

  • For your References section:

    Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells. Miyoshi T, Vishwasrao HD, Belyantseva IA, Miyoshi J, Sajeevadathan M, Ishibashi Y, Adadey SM, Harada N, Shroff H, Friedman TB. Nat Commun. 2025 Sep 1;16(1):8149. doi: 10.1038/s41467-025-63102-0. 10.1038/s41467-025-63102-0 PubMed 40890108
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