pHA-C1
(Plasmid
#251432)
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Purpose(Empty Backbone) Mammalian expression vector to express a protein of interest with an N-terminal HA tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is derived from the Clontech pEGFP-C1 vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHA-C1 was a gift from Takushi Miyoshi (Addgene plasmid # 251432 ; http://n2t.net/addgene:251432 ; RRID:Addgene_251432) -
For your References section:
Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells. Miyoshi T, Vishwasrao HD, Belyantseva IA, Miyoshi J, Sajeevadathan M, Ishibashi Y, Adadey SM, Harada N, Shroff H, Friedman TB. Nat Commun. 2025 Sep 1;16(1):8149. doi: 10.1038/s41467-025-63102-0. 10.1038/s41467-025-63102-0 PubMed 40890108