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pCL400_TETRIS-v3_empty
(Plasmid #251477)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251477 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTETRIS-v3
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains S60G, F97Y, and K131E mutations in luciferase. These mutations are not known to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL400_TETRIS-v3_empty was a gift from Mauro Calabrese (Addgene plasmid # 251477 ; http://n2t.net/addgene:251477 ; RRID:Addgene_251477)

  • For your References section:

    Elements at the 5' end of Xist harbor SPEN-independent transcriptional antiterminator activity. Trotman JB, Lee DM, Cherney RE, Kim SO, Inoue K, Schertzer MD, Bischoff SR, Cowley DO, Calabrese JM. Nucleic Acids Res. 2020 Oct 9;48(18):10500-10517. doi: 10.1093/nar/gkaa789. 10.1093/nar/gkaa789 PubMed 32986830
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