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UPF1-N-term-AID-V5-P2A-Hygro
(Plasmid #251490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251490 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homology arms for N-terminal targeting of UPF1 with AID-Hygro cassette
  • Species
    H. sapiens (human), A. thaliana (mustard weed)
  • Tag / Fusion Protein
    • auxin-induced degron tag (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UPF1-N-term-AID-V5-P2A-Hygro was a gift from Markus Landthaler (Addgene plasmid # 251490 ; http://n2t.net/addgene:251490 ; RRID:Addgene_251490)

  • For your References section:

    Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Boehm V, Wallmeroth D, Wulf PO, Popp O, Teixeira Alves LG, Reinecke L, Riedel M, Wyler E, Franitza M, Becker K, Polkovnychenko K, Del Giudice S, Benlasfer N, Mertins P, Landthaler M, Gehring NH. Mol Cell. 2025 Sep 18;85(18):3524-3546.e12. doi: 10.1016/j.molcel.2025.08.015. Epub 2025 Sep 10. 10.1016/j.molcel.2025.08.015 PubMed 40934927
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