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px458-Cas9 UPF1 sgRNA
(Plasmid #251491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251491 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    px458-Cas9
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UPF1 gRNA
  • gRNA/shRNA sequence
    GCCCGAGGGCCCTACCCGG
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px458-Cas9 UPF1 sgRNA was a gift from Markus Landthaler (Addgene plasmid # 251491 ; http://n2t.net/addgene:251491 ; RRID:Addgene_251491)

  • For your References section:

    Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome. Boehm V, Wallmeroth D, Wulf PO, Popp O, Teixeira Alves LG, Reinecke L, Riedel M, Wyler E, Franitza M, Becker K, Polkovnychenko K, Del Giudice S, Benlasfer N, Mertins P, Landthaler M, Gehring NH. Mol Cell. 2025 Sep 18;85(18):3524-3546.e12. doi: 10.1016/j.molcel.2025.08.015. Epub 2025 Sep 10. 10.1016/j.molcel.2025.08.015 PubMed 40934927
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