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CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA
(Plasmid #251494)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251494 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTwist CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ISCro4 bridge recombinase
  • Species
    Citrobacter rodentium
  • Mutation
    NA

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA was a gift from Martin Jinek (Addgene plasmid # 251494 ; http://n2t.net/addgene:251494 ; RRID:Addgene_251494)

  • For your References section:

    Programmable genome editing in human cells using RNA-guided bridge recombinases. Pelea O, Tálas A, Carrera JF, Mathis N, van de Venn L, Yeh CD, Kulcsár PI, Marquart KF, Weber Y, Gerecke SE, Harvey-Seutcheu IF, Mailänder D, Pfleiderer MM, Chanez C, Corn JE, Schwank G, Jinek M. Science, 2026. eadz1884, doi:10.1126/science.adz1884 10.1126/science.adz1884
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