Tol2_ISCro4_Inversion_Reporter
(Plasmid
#251499)
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PurposeReporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneAddgene #200249
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameISCro4 inversion reporter
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SpeciesSynthetic
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MutationNA
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tol2_ISCro4_Inversion_Reporter was a gift from Martin Jinek (Addgene plasmid # 251499 ; http://n2t.net/addgene:251499 ; RRID:Addgene_251499) -
For your References section:
Programmable genome editing in human cells using RNA-guided bridge recombinases. Pelea O, Tálas A, Carrera JF, Mathis N, van de Venn L, Yeh CD, Kulcsár PI, Marquart KF, Weber Y, Gerecke SE, Harvey-Seutcheu IF, Mailänder D, Pfleiderer MM, Chanez C, Corn JE, Schwank G, Jinek M. Science, 2026. eadz1884, doi:10.1126/science.adz1884 10.1126/science.adz1884