pDonor_ISCro4_(WT)_Donor_ECFP
(Plasmid
#251501)
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PurposeDonor plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and Tol2_ISCro4_Insertion_Reporter plasmid
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepTwist Amp
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameISCro4_(WT)_Donor sequence and ECFP
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SpeciesCitrobacter rodentium
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MutationNA
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonor_ISCro4_(WT)_Donor_ECFP was a gift from Martin Jinek (Addgene plasmid # 251501 ; http://n2t.net/addgene:251501 ; RRID:Addgene_251501) -
For your References section:
Programmable genome editing in human cells using RNA-guided bridge recombinases. Pelea O, Tálas A, Carrera JF, Mathis N, van de Venn L, Yeh CD, Kulcsár PI, Marquart KF, Weber Y, Gerecke SE, Harvey-Seutcheu IF, Mailänder D, Pfleiderer MM, Chanez C, Corn JE, Schwank G, Jinek M. Science, 2026. eadz1884, doi:10.1126/science.adz1884 10.1126/science.adz1884