T7_MBP_TEV_ISCro4_6xHis_T7_term
(Plasmid
#251502)
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PurposeBacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251502 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET-21(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameISCro4 bridge recombinase
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SpeciesCitrobacter rodentium
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MutationNA
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7_MBP_TEV_ISCro4_6xHis_T7_term was a gift from Martin Jinek (Addgene plasmid # 251502 ; http://n2t.net/addgene:251502 ; RRID:Addgene_251502) -
For your References section:
Programmable genome editing in human cells using RNA-guided bridge recombinases. Pelea O, Tálas A, Carrera JF, Mathis N, van de Venn L, Yeh CD, Kulcsár PI, Marquart KF, Weber Y, Gerecke SE, Harvey-Seutcheu IF, Mailänder D, Pfleiderer MM, Chanez C, Corn JE, Schwank G, Jinek M. Science, 2026. eadz1884, doi:10.1126/science.adz1884 10.1126/science.adz1884