pCS05
(Plasmid #251507)

• Purpose: Integrase expression, ФC31 (marker curing / backbone excision)
• Depositing Lab: James Carothers
• Publication: Conjugation-based Serine Recombinase-Assisted Genome Engineering (cSAGE) Enables Bioproduction in Non-model Bacteria (unpublished)

Backbone

• Vector backbone: pR6K
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PhiC31 integrase

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Elmore, Joshua R., et al. "High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration." Science Advances 9.10 (2023): eade1285.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternative name: pSG010

Usage/Comments: This plasmid expresses ΦC31 integrase and is used for marker curing and plasmid backbone excision after payload integration. When payload plasmids are designed with ΦC31 att sites flanking the selectable marker and backbone, introduction of this plasmid by conjugation catalyzes recombination between att sites, excising the antibiotic resistance cassette and vector backbone. This enables generation of markerless strains suitable for downstream engineering cycles or deployment. Counterselection (e.g., sacB-based sucrose selection) may be used depending on the payload design. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.