pAcBAc1-Ma-tfmW-RSA2
(Plasmid
#251519)
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PurposeOrthogonal Methanomethylophilus alvus aaRS/tRNA-mediated incorporation of tfm-Tryptophan into sfGFP150TAG in HEK293T cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAcBAc1-Ma
- Backbone size w/o insert (bp) 9010
- Total vector size (bp) 9488
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMa-tfmW-RSA2
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SpeciesSynthetic
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Insert Size (bp)834
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer gagacccaagctggctagcgccaccatgacagtgaa
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcBAc1-Ma-tfmW-RSA2 was a gift from Ryan Mehl (Addgene plasmid # 251519 ; http://n2t.net/addgene:251519 ; RRID:Addgene_251519) -
For your References section:
Endogenous Site-Specific Encoding of Trifluoromethyl-Bearing Phenylalanine and Tryptophan for in-Cell (19)F NMR. Augustin G, Bhinderwala F, Alexander ND, Hernandez I, Stanisheuski S, Monnie CM, Eddins AJ, Gangarde YM, Soloshonok VA, Oiarbide M, Landa A, Cooley RB, Gronenborn AM, Mehl RA. J Am Chem Soc. 2026 Feb 12. doi: 10.1021/jacs.5c18349. 10.1021/jacs.5c18349 PubMed 41684198