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ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY)
(Plasmid #251547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251547 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ecTadA(8e)_V28C_M151E-nCas9_SpRY
  • Species
    Synthetic
  • Mutation
    ABEx4 V28C_M151E

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY) was a gift from George Church (Addgene plasmid # 251547 ; http://n2t.net/addgene:251547 ; RRID:Addgene_251547)

  • For your References section:

    Engineered base editors with reduced bystander editing through directed evolution. Perrotta RM, Vinke S, Ferreira R, Moret M, Mahas A, Chiappino-Pepe A, Riedmayr LM, Mehra AT, Lehmann LS, Church GM. Nat Biotechnol. 2025 Dec 18. doi: 10.1038/s41587-025-02937-w. 10.1038/s41587-025-02937-w PubMed 41413244
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