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PACT wild type
(Plasmid #251697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251697 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET47(b)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRKRA
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRKRA (a.k.a. DYT16, HSD14, PACT, RAX)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PACT wild type was a gift from Sun Hur (Addgene plasmid # 251697 ; http://n2t.net/addgene:251697 ; RRID:Addgene_251697)

  • For your References section:

    PACT prevents aberrant activation of PKR by endogenous dsRNA without sequestration. Ahmad S, Zou T, Hwang J, Zhao L, Wang X, Davydenko A, Buchumenski I, Zhuang P, Fishbein AR, Capcha-Rodriguez D, Orgel A, Levanon EY, Myong S, Chou J, Meyerson M, Hur S. Nat Commun. 2025 Apr 8;16(1):3325. doi: 10.1038/s41467-025-58433-x. 10.1038/s41467-025-58433-x PubMed 40199855
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