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pVAM03
(Plasmid #251701)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251701 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pKEK2201
  • Backbone size (bp) 5888
  • Modifications to backbone
    Removal of aph(3′) gene (kanamycin resistance) and insertion of Tetracycline resistance gene
  • Vector type
    Sucrose counter-selection allelic exchange vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Other

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Vector backbone pKEK2201 (Mejia-Santana et al. 2021, PMID: 33492170). Tetracycline resistance marker derived from pBBR1MCS-3 (Kovach et al. 1995, PMID: 8529885).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVAM03 was a gift from James Bina (Addgene plasmid # 251701 ; http://n2t.net/addgene:251701 ; RRID:Addgene_251701)

  • For your References section:

    New vectors and optimal conditions for allelic exchange in hypervirulent Klebsiella pneumoniae. Van Allen ME, Chandrashekarappa DG, Bina XR, Bina JE. J Microbiol Methods. 2025 Jan;228:107070. doi: 10.1016/j.mimet.2024.107070. Epub 2024 Nov 19. 10.1016/j.mimet.2024.107070 PubMed 39566662
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