pSF-aTubCaud-FLAG-GCaMP8s
(Plasmid
#251708)
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PurposeParamecium caudatum codon-optimized GCaMP8s fluorescent reporter for calcium signaling
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSF
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Backbone manufacturerIlya Skovorodkin and Seppo Vainio
- Backbone size w/o insert (bp) 4022
- Total vector size (bp) 5279
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Modifications to backbonePlasmid linearized through ApaI to generate linear product for transformation into P. caudatum
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Vector typeProtozoan Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP8s
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SpeciesP. caudatum
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Insert Size (bp)1257
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer whole-plasmid sequencing
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byWhole-plasmid synthesis by Twist
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSF-aTubCaud-FLAG-GCaMP8s was a gift from Daniel Fürth (Addgene plasmid # 251708 ; http://n2t.net/addgene:251708 ; RRID:Addgene_251708)