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pSF-aTubCaud-FLAG-GCaMP8s
(Plasmid #251708)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251708 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pSF
  • Backbone manufacturer
    Ilya Skovorodkin and Seppo Vainio
  • Backbone size w/o insert (bp) 4022
  • Total vector size (bp) 5279
  • Modifications to backbone
    Plasmid linearized through ApaI to generate linear product for transformation into P. caudatum
  • Vector type
    Protozoan Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP8s
  • Species
    P. caudatum
  • Insert Size (bp)
    1257
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF-aTubCaud-FLAG-GCaMP8s was a gift from Daniel Fürth (Addgene plasmid # 251708 ; http://n2t.net/addgene:251708 ; RRID:Addgene_251708)