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CAG-LifeCamp
(Plasmid #251723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251723 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4848
  • Total vector size (bp) 6957
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LifeCamp
  • Species
    Synthetic
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atgcatcatcaccatcatcacacgc
  • 3′ sequencing primer tttatagagttcgtccattcccaatgt
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.23.696288 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-LifeCamp was a gift from Bernardo Sabatini (Addgene plasmid # 251723 ; http://n2t.net/addgene:251723 ; RRID:Addgene_251723)

  • For your References section:

    Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements. Lodder B, Raghubardayal M, Ganesh S, Cai X, Stern J, Sherman M, Rosen P, Kamath T, Hartman I, Siegel M, Timmins J, Adan R, Andermann M, Sabatini BL. bioRxiv 2025.12.23.696288 10.64898/2025.12.23.696288
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