pAAV-hsyn-RiboL1-LifeCamp
(Plasmid #251728)

• Purpose: Expression of soma-targeted calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone
• Depositing Lab: Bernardo Sabatini
• Publication: Lodder et al bioRxiv 2025.12.23.696288

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3834
• Total vector size (bp): 6675
• Modifications to backbone: Replacement of WPRE and hGH polyA sequence with shorter WPRE3 and SV40 late polyA sequence to fit within AAV packaging constraints
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RiboL1-LifeCamp
• Species: Synthetic
• Insert Size (bp): 2841

Resource Information

• Supplemental Documents:
  • hSyn-lifecamp-ribol1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.23.696288 for bioRxiv preprint.