pGEF-U
(Plasmid
#251731)
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Purpose(Empty Backbone) Geminivirus derived-CRISPR/Cas9 plasmid with GFP for cloning of up to four gRNAs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGMV-U
- Backbone size (bp) 16816
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Modifications to backboneAddition of eGFP under the control of CaMV35S promoter
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEF-U was a gift from Humberto Prieto (Addgene plasmid # 251731)