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pGL4-hACLY promoter
(Plasmid #251827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251827 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Total vector size (bp) 5200
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACLY promoter
  • Alt name
    hg38; chr17: 41918547-41919547
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1007
  • Entrez Gene
    ACLY (a.k.a. ACL, ATPCL, CLATP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4-hACLY promoter was a gift from Daniel Herranz (Addgene plasmid # 251827 ; http://n2t.net/addgene:251827 ; RRID:Addgene_251827)

  • For your References section:

    A feedforward loop between ACLY and MYC supports T-ALL progression in vivo. da Silva-Diz V, Singh A, Aleksandrova M, Kim O, Thai C, Lancho O, Renck Nunes P, Affronti H, Martinez de Paz A, Josefowicz SZ, Su X, Wellen KE, Herranz D. Blood Neoplasia. 2025 Jan 20;2(2):100069. doi: 10.1016/j.bneo.2025.100069. eCollection 2025 May. 10.1016/j.bneo.2025.100069 PubMed 40453140
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