pGL2_340
(Plasmid
#251920)
-
PurposeLevel 2 plasmid, barcoded ORI with spectinomycin resistance cassette and mScarlet-I reporter
-
Depositing Labs
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251920 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonen/a
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Pir1
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepAL5000 ORI plus NGS barcode 1, spectinomycin resistance cassette, mScarlet-I cassette, oriT
-
SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may run as a dimer or higher multimer.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL2_340 was a gift from Charlie Gilbert & Nili Ostrov & Cultivarium Tools (Addgene plasmid # 251920 ; http://n2t.net/addgene:251920 ; RRID:Addgene_251920) -
For your References section:
Design and construction towards a pan-microbial toolkit. Gilbert C, Crits-Christoph A, Ledieu-Dherbécourt E, Kang SC, Brumwell SL, Lee HH, Nili Ostrov N. bioRxiv 2024.02.23.581749 10.1101/2024.02.23.581749