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pADH1-MTS-ABE8e-P2A-EGFP
(Plasmid #251973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251973 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAG415
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE8e
  • Species
    S. cerevisiae (budding yeast)
  • Promoter ADH1
  • Tags / Fusion Proteins
    • MTS (N terminal on insert)
    • V5-P2A-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pADH1-MTS-ABE8e-P2A-EGFP was a gift from Alice Ting (Addgene plasmid # 251973 ; http://n2t.net/addgene:251973 ; RRID:Addgene_251973)

  • For your References section:

    Towards CRISPR-based editing of the mitochondrial genome in yeast. Yin S, Jarosz DF, Ting AY. Proc Natl Acad Sci U S A. 2026 Jan 20;123(3):e2505894123. doi: 10.1073/pnas.2505894123. Epub 2026 Jan 16. 10.1073/pnas.2505894123 PubMed 41543903
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