Skip to main content
Addgene
Open Menu

pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV
(Plasmid #251982)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251982 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pPT24
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mtArgm*
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    premature stop CAA->TAA, Q79*
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Gene/Insert 2

  • Gene/Insert name
    HH-nt sgRNA-HDV

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert is flanked by the 5' and 3'UTR of COX2 and the complete COX2 gene further downstream. Please visit https://doi.org/10.64898/2025.12.09.693232 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV was a gift from Alice Ting (Addgene plasmid # 251982 ; http://n2t.net/addgene:251982 ; RRID:Addgene_251982)

  • For your References section:

    Towards CRISPR-based editing of the mitochondrial genome in yeast. Yin S, Jarosz DF, Ting AY. Proc Natl Acad Sci U S A. 2026 Jan 20;123(3):e2505894123. doi: 10.1073/pnas.2505894123. Epub 2026 Jan 16. 10.1073/pnas.2505894123 PubMed 41543903
Related items: