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pBTK1212
(Plasmid #251993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251993 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBBR1
  • Vector type
    Bacterial Expression ; Biosensor

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Split mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)
  • Promoter PBAD

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.18.700149 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK1212 was a gift from Jeffrey Barrick (Addgene plasmid # 251993 ; http://n2t.net/addgene:251993 ; RRID:Addgene_251993)

  • For your References section:

    Genetic biosensor for optimizing double-stranded RNA production by bacterial symbionts. Navarro-Escalante L, VanDieren AJ, Barrick JE. bioRxiv 2026.01.18.700149 10.64898/2026.01.18.700149
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