pBTK1112 (pDS-GFP<T7)
(Plasmid
#251995)
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PurposeExpresses double-stranded RNA targeting the GFP gene, under control of T7 promoters.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251995 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneRSF1010
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Vector typeControl
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFPmut3 fragment
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Insert Size (bp)717
- Promoter T7 (no RBS)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer N/A
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.18.700149 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK1112 (pDS-GFP<T7) was a gift from Jeffrey Barrick (Addgene plasmid # 251995 ; http://n2t.net/addgene:251995 ; RRID:Addgene_251995) -
For your References section:
Genetic biosensor for optimizing double-stranded RNA production by bacterial symbionts. Navarro-Escalante L, VanDieren AJ, Barrick JE. bioRxiv 2026.01.18.700149 10.64898/2026.01.18.700149
