Skip to main content
Addgene
Open Menu

pTE_02 - pLenti_EFS-Cas9-XTEN-FAN1-2A-BlastR
(Plasmid #251998)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251998 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    lentiCRISPRv2
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-FAN1
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    NA

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.02.19.706875 for bioRxiv preprint.

A G232E mutation was found in FAN1 and does not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE_02 - pLenti_EFS-Cas9-XTEN-FAN1-2A-BlastR was a gift from Neville Sanjana (Addgene plasmid # 251998 ; http://n2t.net/addgene:251998 ; RRID:Addgene_251998)

  • For your References section:

    Rational design of synthetic proteins using a genome-scale CRISPR screen. Burrell WH, Mueller SJ, Daniloski Z, Duffy Doyle Jr. P, Rovsing AB, James C, Drabkin M, Chou C-Y, So HYA, Katgara L, Sookdeo A, Lu L, Cisse G-I, Yan RE, Sanjana NE. bioRxiv 2026.02.19.706875 10.64898/2026.02.19.706875
Related items: