pTE_23 - pDonor_TRAC-2a-FMC6.3-BBz-2a
(Plasmid
#252019)
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PurposeDonor for CD19 CAR knock-in at TRAC locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonePUC19
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2a-FMC6.3-BBz-2a
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SpeciesH. sapiens (human), Synthetic
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MutationNA
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTE_23 - pDonor_TRAC-2a-FMC6.3-BBz-2a was a gift from Neville Sanjana (Addgene plasmid # 252019 ; http://n2t.net/addgene:252019 ; RRID:Addgene_252019)