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erCRY1b crystal
(Plasmid #252022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252022 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pfastbac Htb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6230
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    European robin cry1b
  • Species
    europen robin
  • Insert Size (bp)
    1530
  • GenBank ID
    KT380949
  • Promoter p10
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CCTATAAATATTCCGGATTATTCATACC
  • 3′ sequencing primer ACAAATGTGGTATGGCTGA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    erCRY1b crystal was a gift from Joseph Takahashi (Addgene plasmid # 252022 ; http://n2t.net/addgene:252022 ; RRID:Addgene_252022)

  • For your References section:

    Structure of European robin cryptochrome 1 reveals a role in circadian rhythms, not magnetoreception. Wickramaratne AC, Rasmussen ES, Chelliah Y, Schuhmann F, Solov'yov IA, Mouritsen H, Green CB, Zoltowski BD, Takahashi JS. iScience. 2025 Nov 11;28(12):114015. doi: 10.1016/j.isci.2025.114015. eCollection 2025 Dec 19. 10.1016/j.isci.2025.114015 PubMed 41488359
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