rtTA-PuroR, Mito-EGFP-OMM-PINK1-PRKN
(Plasmid
#252078)
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PurposeA PiggyBac donor vector that allows for the Dox-inducible expression of mito-EGFP, OMM-PINK1, and PRKN for enforced mitophagy in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBR322
- Total vector size (bp) 11310
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Vector typeMammalian Expression ; PiggyBac transposon donor vector
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rtTA-PuroR, Mito-EGFP-OMM-PINK1-PRKN was a gift from Jun Wu (Addgene plasmid # 252078 ; http://n2t.net/addgene:252078 ; RRID:Addgene_252078) -
For your References section:
Unraveling mitochondrial influence on mammalian pluripotency via enforced mitophagy. Schmitz DA, Oura S, Li L, Ding Y, Dahiya R, Ballard E, Pinzon-Arteaga C, Sakurai M, Okamura D, Yu L, Ly P, Wu J. Cell. 2025 Aug 21;188(17):4773-4789.e22. doi: 10.1016/j.cell.2025.05.020. Epub 2025 Jun 10. 10.1016/j.cell.2025.05.020 PubMed 40499542