Skip to main content

rtTA-HygR-Mito-EGFP, OMM-PINK1-PuroR-PRKN
(Plasmid #252080)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252080 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pBR322
  • Total vector size (bp) 13410
  • Vector type
    Mammalian Expression ; PiggyBac transposon donor vector
  • Selectable markers
    Hygromycin ; PuroR under the control of tight TRE promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mito-EGFP, OMM-PINK1, PRKN
  • Species
    H. sapiens (human)
  • Entrez Gene
    PINK1 (a.k.a. BRPK, PARK6)
  • Entrez Gene
    PRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rtTA-HygR-Mito-EGFP, OMM-PINK1-PuroR-PRKN was a gift from Jun Wu (Addgene plasmid # 252080 ; http://n2t.net/addgene:252080 ; RRID:Addgene_252080)
  • For your References section:

    Unraveling mitochondrial influence on mammalian pluripotency via enforced mitophagy. Schmitz DA, Oura S, Li L, Ding Y, Dahiya R, Ballard E, Pinzon-Arteaga C, Sakurai M, Okamura D, Yu L, Ly P, Wu J. Cell. 2025 Aug 21;188(17):4773-4789.e22. doi: 10.1016/j.cell.2025.05.020. Epub 2025 Jun 10. 10.1016/j.cell.2025.05.020 PubMed 40499542