pRembiv
(Plasmid #252216)

• Purpose: Yeast expression of BK polyomavirus VP1 and aeBlue reporter
• Depositing Lab: Christopher Buck
• Publication: An Edible Polyomavirus Vaccine (unpublished)

Backbone

• Vector backbone: pGustiv
• Backbone manufacturer: Gusteau Research Corporation
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 9165
• Vector type: Yeast Expression
• Selectable markers: formaldehyde resistance

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BKV-IV VP1
• Species: BK polyomavirus
• Insert Size (bp): 1089
• GenBank ID: BAG84476
• Promoter: MAL32

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: Plasmidsaurus

Gene/Insert 2

• Gene/Insert name: aeBlue
• Species: Actinia equina
• Insert Size (bp): 696
• GenBank ID: DQ159069
• Promoter: MAL32

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: Plasmidsaurus

Gene/Insert 3

• Gene/Insert name: Formaldehyde dehydrogenase 1
• Species: Candida maltosa
• Insert Size (bp): 1146
• GenBank ID: EMG49224
• Promoter: FDH1

Cloning Information for Gene/Insert 3

• Cloning method: Gene Synthesis
• 5′ sequencing primer: Plasmidsaurus

Resource Information

• Supplemental Documents:
  • pRembiv.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • OpenMTA
• Industry Terms:
  • OpenMTA