pSW-Apr
(Plasmid
#252219)
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PurposeExpresses the endonuclease SceI to induce recombination. Carries an apramycin resistance gene.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSW-Apr
- Total vector size (bp) 8722
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Modifications to backboneThis plasmid is a modified version of pSW-I (Martinez-Garcia & de Lorenzo, 2011) with the addition of an apramycin resistance gene in the ScaI site.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 50–100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSW-Apr was a gift from Ronan McCarthy (Addgene plasmid # 252219 ; http://n2t.net/addgene:252219 ; RRID:Addgene_252219) -
For your References section:
A high-efficiency scar-free genome-editing toolkit for Acinetobacter baumannii. de Dios R, Gadar K, McCarthy RR. J Antimicrob Chemother. 2022 Nov 28;77(12):3390-3398. doi: 10.1093/jac/dkac328. 10.1093/jac/dkac328 PubMed 36216579