ePB-Puro_TT-hGATA3-P2A-tdTomato-CAAX
(Plasmid
#252250)
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PurposeA PiggyBac donor vector for Dox-inducible expression of GATA3 and membrane-localized tdTomato.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneSKII
- Total vector size (bp) 9786
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Vector typeMammalian Expression ; PiggyBac transposon donor vector
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGATA3
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SpeciesH. sapiens (human)
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Entrez GeneGATA3 (a.k.a. HDR, HDRS)
- Promoter tight TRE
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Tag
/ Fusion Protein
- P2A-tdTomato-CAAX (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ePB-Puro_TT-hGATA3-P2A-tdTomato-CAAX was a gift from Jun Wu (Addgene plasmid # 252250 ; http://n2t.net/addgene:252250 ; RRID:Addgene_252250) -
For your References section:
An inducible model of human post-implantation development derived from primed and naive stem cells. Oura S, Li L, Wu J. Cell Stem Cell. 2025 Oct 2;32(10):1509-1527.e9. doi: 10.1016/j.stem.2025.08.005. Epub 2025 Aug 29. 10.1016/j.stem.2025.08.005 PubMed 40885193