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ePB-Puro_TT-hYAP-5SA-P2A-tdTomato-CAAX
(Plasmid #252251)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252251 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    SKII
  • Total vector size (bp) 9913
  • Vector type
    Mammalian Expression ; PiggyBac transposon donor vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YAP-5SA
  • Species
    H. sapiens (human)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter tight TRE
  • Tag / Fusion Protein
    • P2A-tdTomato-CAAX (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ePB-Puro_TT-hYAP-5SA-P2A-tdTomato-CAAX was a gift from Jun Wu (Addgene plasmid # 252251 ; http://n2t.net/addgene:252251 ; RRID:Addgene_252251)

  • For your References section:

    An inducible model of human post-implantation development derived from primed and naive stem cells. Oura S, Li L, Wu J. Cell Stem Cell. 2025 Oct 2;32(10):1509-1527.e9. doi: 10.1016/j.stem.2025.08.005. Epub 2025 Aug 29. 10.1016/j.stem.2025.08.005 PubMed 40885193
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