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pGEX4T-2 GST-mouse Fis1 (ΔTM domain)
(Plasmid #252268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252268 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEX4T-2
  • Backbone size w/o insert (bp) 4279
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Fis1 (deltaTM domain)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1044
  • Mutation
    moue Fis1 1-121 amino acids
  • Entrez Gene
    Fis1 (a.k.a. 2010003O14Rik, Ttc11)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI, SalI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid expresses a GST-tagged N-terminal fragment of mouse Fis1 (1-121 AAs) lacking the C-terminal TM domain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-2 GST-mouse Fis1 (ΔTM domain) was a gift from David Chan (Addgene plasmid # 252268 ; http://n2t.net/addgene:252268 ; RRID:Addgene_252268)
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