pE-SUMO_PspCas13b
(Plasmid
#252295)
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PurposeExpresses PspCas13b (WT) in bacterial cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepE-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePspCas13b (WT)
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SpeciesPrevotella sp.
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MutationWT
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pE-SUMO_PspCas13b was a gift from Hiroshi Nishimasu (Addgene plasmid # 252295 ; http://n2t.net/addgene:252295 ; RRID:Addgene_252295) -
For your References section:
Structural insights into RNA-guided RNA editing by the Cas13b-ADAR2 complex. Ishikawa J, Kato K, Kannan S, Okazaki S, Ishiguro S, Yamashita K, Yachie N, Nishizawa T, Zhang F, Nishimasu H. Nat Struct Mol Biol. 2025 Aug;32(8):1567-1578. doi: 10.1038/s41594-025-01529-1. Epub 2025 Apr 11. 10.1038/s41594-025-01529-1 PubMed 40217120