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pFastBac-WT-REPAIR
(Plasmid #252296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252296 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    REPAIR (WT)
  • Alt name
    dPspCas13b–ADAR2DD fusion
  • Species
    H. sapiens (human); Prevotella sp.
  • Mutation
    dPspCas13b H133A/H1058A; ADAR2DD E488Q

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-WT-REPAIR was a gift from Hiroshi Nishimasu (Addgene plasmid # 252296 ; http://n2t.net/addgene:252296 ; RRID:Addgene_252296)

  • For your References section:

    Structural insights into RNA-guided RNA editing by the Cas13b-ADAR2 complex. Ishikawa J, Kato K, Kannan S, Okazaki S, Ishiguro S, Yamashita K, Yachie N, Nishizawa T, Zhang F, Nishimasu H. Nat Struct Mol Biol. 2025 Aug;32(8):1567-1578. doi: 10.1038/s41594-025-01529-1. Epub 2025 Apr 11. 10.1038/s41594-025-01529-1 PubMed 40217120
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