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pACYCDuet-1-Eco7-ncRNA
(Plasmid #252301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252301 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACYC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Retron-Eco7 non-coding RNA
  • Species
    Escherichia coli
  • Mutation
    WT

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYCDuet-1-Eco7-ncRNA was a gift from Hiroshi Nishimasu (Addgene plasmid # 252301 ; http://n2t.net/addgene:252301 ; RRID:Addgene_252301)

  • For your References section:

    Structural mechanism of the Retron-Eco7 anti-phage defense system. Ishikawa J, Yoneyama K, Azam AH, Nagao A, Mitsuda Y, Nakazaki R, Chihara K, Hiraizumi M, Yamashita K, Suzuki T, Kiga K, Nishimasu H. Nat Commun. 2025 Dec 2;16(1):10821. doi: 10.1038/s41467-025-66589-9. 10.1038/s41467-025-66589-9 PubMed 41330933
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