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TP1321
(Plasmid #252360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252360 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDG-Kan
  • Backbone manufacturer
    Dirk Görlich Lab
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-EMC nanobody E4
  • Species
    Alpaca (Vicugna pacos)
  • Insert Size (bp)
    1548
  • Mutation
    Contains engineered nanobody variant with ALFA tag, sfGFP, SUMOEu fusion, and His10 tag.
  • Tags / Fusion Proteins
    • ALFA-sfGFP-SUMOEu (N terminal on insert)
    • His10 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.14.699575 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TP1321 was a gift from Tino Pleiner (Addgene plasmid # 252360 ; http://n2t.net/addgene:252360 ; RRID:Addgene_252360)

  • For your References section:

    The ER membrane protein complex acts as a chaperone to promote the biogenesis of multi-bundle membrane proteins. Stanton M, Singal B, Biswal M, Agarwal M, Scheuing CE, Vargas GD, Gao A, Gifford CA, Pleiner T. bioRxiv [Preprint]. 2026 Jan 15:2026.01.14.699575. doi: 10.64898/2026.01.14.699575. 10.64898/2026.01.14.699575 PubMed 41648177
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