pCMV_aActTS_indicator
(Plasmid
#252385)
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PurposeNon-FRET aActinin tension indicator. cpEGFP with elastic linker is inserted at the hinge with C-term mCherry.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-N1 derived backbone (Kan/Neo)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaActinin tension indicator
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_aActTS_indicator was a gift from Maretoshi Hirai (Addgene plasmid # 252385 ; http://n2t.net/addgene:252385 ; RRID:Addgene_252385) -
For your References section:
Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs. Fujiwara K, Fujiki K, Akama TO, Shirahige K, Shiojima I, Nakamura T, Hirai M. Commun Biol. 2026 Feb 19. doi: 10.1038/s42003-026-09746-0. 10.1038/s42003-026-09746-0 PubMed 41714452