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pCL1009
(Plasmid #252430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252430 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    peGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H3.1
  • Species
    H. sapiens (human)
  • Mutation
    Truncation: aa 1-30 only
  • Entrez Gene
    H3C1 (a.k.a. H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP-TVMVx2-FRB-OMP25 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL1009 was a gift from Andrew Bowman (Addgene plasmid # 252430 ; http://n2t.net/addgene:252430 ; RRID:Addgene_252430)

  • For your References section:

    Evidence for the nuclear import of histones H3.1 and H4 as monomers. Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ. EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3. 10.15252/embj.201798714 PubMed 30177573
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