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AAV-GFABC1D-GJA1-GGGGS-TID-HA
(Plasmid #252501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252501 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 2619
  • Total vector size (bp) 6654
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GJA1
  • Alt name
    Cx43
  • Alt name
    Connexin 43
  • Alt name
    Gap Junction A1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1146
  • Mutation
    deleted stop codon
  • GenBank ID
    NM_001428498.1
  • Entrez Gene
    Gja1 (a.k.a. Cnx43, Cx43, Cx43alpha1, Cxnk1, Gja-1, Npm1, connexin43)
  • Tags / Fusion Proteins
    • 3xGGGGS
    • TurboID
    • HA (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    GFABC1D
  • Alt name
    Shortened GFAP Promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    681

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vector Builder

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.18.665573 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GFABC1D-GJA1-GGGGS-TID-HA was a gift from Moses Chao (Addgene plasmid # 252501 ; http://n2t.net/addgene:252501 ; RRID:Addgene_252501)

  • For your References section:

    Astrocytes connect specific brain regions through plastic networks. Cooper ML, Selles MC, Cammer M, Redd C, Gildea HK, Sall J, Chiurri KE, Cheung P, Wheeler DG, Saab AS, Liddelow SA, Chao MV. Nature (2026). doi: https://doi.org/10.1038/s41586-026-10426-6 10.1038/s41586-026-10426-6
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