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pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR
(Plasmid #252529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252529 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pZR071_SFFV-dCas9-mCherry-KRAB
  • Backbone manufacturer
    Alexander Marson, Addgene plasmid #180264
  • Total vector size (bp) 13313
  • Modifications to backbone
    Replaced the dCas9-KRAB with ZIM3-dCas9-2A-BsdR
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZIM3-dCas9-P2A-BsdR
  • Promoter EF1a

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was cloned by Zachary Steinhart.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.23.696273 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR was a gift from Alexander Marson (Addgene plasmid # 252529 ; http://n2t.net/addgene:252529 ; RRID:Addgene_252529)

  • For your References section:

    Genome-scale perturb-seq in primary human CD4(+) T cells maps context-specific regulators of T cell programs and human immune traits. Zhu R, Dann E, Yan J, Reyes Retana J, Goto R, Guitche RC, Brixi L, Ota M, Hartman A, Roth TL, Satpathy AT, Pritchard JK, Marson A. Cell. 2026 Aug 28:S0092-8674(26)00929-3. doi: 10.1016/j.cell.2026.08.002. 10.1016/j.cell.2026.08.002 PubMed 42664972
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