pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR
(Plasmid
#252529)
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PurposeExpress ZIM3-dCas9 and BSD
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepZR071_SFFV-dCas9-mCherry-KRAB
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Backbone manufacturerAlexander Marson, Addgene plasmid #180264
- Total vector size (bp) 13313
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Modifications to backboneReplaced the dCas9-KRAB with ZIM3-dCas9-2A-BsdR
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZIM3-dCas9-P2A-BsdR
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe plasmid was cloned by Zachary Steinhart.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid was cloned by Zachary Steinhart.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR was a gift from Alexander Marson (Addgene plasmid # 252529 ; http://n2t.net/addgene:252529 ; RRID:Addgene_252529)
