Alexander Marson Lab: Genome-scale perturb-seq in primary human CD4+ T cells maps context-specific regulators of T cell programs and human immune traits
Zhu R, Dann E, Yan J, Retana JR, Goto R, Guitche RC, Petersen LK, Ota M, Pritchard JK, Marson A
bioRxiv 2025.12.23.696273
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251159 | lentiGuide-Puro-PJY390 | Cloning backbone for guide RNAs compatible with 10x FLEX |
| 251161 | lentiGuide-Puro-P2A-EGFP-PJY392 | Cloning backbone for guide RNAs compatible with 10x FLEX |
| 252529 | pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR | Express ZIM3-dCas9 and BSD |
| 254300 | CROPseq-Puro-P2A-EGFP-PJY463 | Cloning backbone for guide RNAs compatible with 10x FLEX and CROPseq |