Genome-scale perturb-seq in primary human CD4(+) T cells maps context-specific regulators of T cell programs and human immune traits.
Zhu R, Dann E, Yan J, Reyes Retana J, Goto R, Guitche RC, Brixi L, Ota M, Hartman A, Roth TL, Satpathy AT, Pritchard JK, Marson A
Cell. 2026 Aug 28:S0092-8674(26)00929-3. doi: 10.1016/j.cell.2026.08.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251159 | lentiGuide-Puro-PJY390 | Cloning backbone for guide RNAs compatible with 10x FLEX |
| 251161 | lentiGuide-Puro-P2A-EGFP-PJY392 | Cloning backbone for guide RNAs compatible with 10x FLEX |
| 252529 | pZR316_Lenti-Ef1a-ZIM3-dCas9-2A-BsdR | Express ZIM3-dCas9 and BSD |
| 254300 | CROPseq-Puro-P2A-EGFP-PJY463 | Cloning backbone for guide RNAs compatible with 10x FLEX and CROPseq |
| 254479 | htCRISPRi-Puro | Genome-scale CRISPRi library for inhibiting transcription of expressed genes in human T cell with 2 sgRNAs/gene; expresses puromycin resistance gene. |
| 254480 | htCRISRPi-Puro-GFP | Genome-scale CRISPRi library for inhibiting transcription of expressed genes in human T cell with 2 sgRNAs/gene; expresses both puromycin resistance gene and GFP. |